Western blots confirmed the presence of mt APEX and mt Bir A* proteins of the expected mass (Figure 1C–D).
The mitochondrion of apicomplexan parasites is critical for parasite survival, although the full complement of proteins that localize to this organelle has not been defined.
Here we undertake two independent approaches to elucidate the mitochondrial proteome of the apicomplexan Toxoplasma gondii.
Apicomplexans belong to a group of eukaryotes known as the myzozoans, that also include dinoflagellates and chromerids (Cavalier-Smith and Chao, 2004).
Chromerids are a phylum of free-living, photosynthetic eukaryotes, and are thought to be the closest extant relatives of apicomplexans (Moore et al., 2008).
Mitochondria were derived from α-proteobacteria in an endosymbiotic event, and were present in the last common ancestor of all eukaryotes (Gray, 2012; Sagan, 1967).